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Biokine Therapeutics
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Image Search Results
Journal: Cell Death & Disease
Article Title: Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment
doi: 10.1038/cddis.2013.88
Figure Lengend Snippet: Characterization of HS-181 cell line after treatment with zebularine. ( a ) RT-PCR of cardiac markers after treatment with zebularine. Myh7, Myh6, Actc, cTnI and Serca2 present more expression after treatment. ( b ) Immunostaining of treated cells to detect cardiac-specific proteins. Scale bars; 50 μ m. ( c ) Blots of Proteome Profiler Array and the resulting quantification histograms demonstrating inhibition of pluripotency marker expression and ( d ) increased levels of mesodermic proteins after zebularine treatment (black arrows)
Article Snippet: Protein expression profiles were assayed using the specific human pluripotent Stem Cell array kit
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Inhibition, Marker